Diabetes has become a disease now affecting the normal human life. Generally, the concentration of glucose inside human bodies is 3-8mM, and the concentration of glucose in the body of patients with diabetes is 9-40mM. Glucose concentration is a key indicator of diagnosis of diabetes, there are already a lot of testing methods for detecting the concentration of dextrose, common are: high performance liquid chromatography, electrochemistry, colorimetry and the like. Wherein the colorimetric method in comparison with the other due to high sensitivity, easy operation and low cost by more people's attention. The glucose sensor is based on a large number of horseradish peroxidase assay set up, however, limited sources of natural enzymes, purification difficult, expensive, and in order to maintain its cleaning, the experimental conditions and operating environment requirements are more demanding , its application is extremely limited, so the development and application of enzyme mimics more and more people's attention.

 

Since the Fe₃O₄ nanoparticles are found to have catalytic properties similar to horseradish peroxidase, people have developed a series of functional classes peroxidase nanomaterials. Such enzyme having nanometer analog preparation process is simple, easy to storage conditions, low cost, high temperature and acid, etc., has become the object of focus on the people, and therefore continue to discover new catalytic activity contain nanomaterials, explore new nano analog enzyme is an important direction of current research.Through our preliminary investigation we found that many have photothermal effect nanomaterials such Fe₃CVCuS, Prussian blue, Au and so has the nature of nanometer analog enzyme. Cs_xWO₃ as photothermal material, according to the near-infrared at having before Chong shenGuo on Nanoscale et al reported a high absorption and a high conversion rate, and therefore can be used for cancer photothermal therapy. Based on these studies we believe Cs_xWO₃ nanorods may also have catalytic activity and enzyme applications like simulation, this idea was first proposed and validated.

 

 

 

The present invention thus provides in one kind Cs_xWO₃ class and mimetic peroxidase and its preparation method and application. Cs_xWO₃ nanomaterials can catalyze H₂O₂ to produce strong oxidizing hydroxyl radicals, highly efficient oxidation of 3,3 ', 5,5' - tetramethylbenzidine (TMB) produces color reaction. Short reaction time, color-fast, catalytic effect and applicability are higher than the natural peroxidase, and can be used in blood glucose levels in food testing.

 

The process according to Cs_xWO₃ class mimetic peroxidase is: will WCl₆ dissolved with stirring in absolute ethanol was added CsOH.H₂O, dispersed evenly, then add anhydrous acetic acid; the resulting mixed solution was transferred to Teflon autoclave, 235 ° C reaction 20h; centrifugal washing, washed with ethanol several times, and then at 60 ° C under vacuum to obtain dried 12h Cs_xWO₃ nanomaterials.

 

More specifically: slightly yellow 0.2976g WCl₆ WCl₆ ethanol solution under vigorous stirring until dissolved in 40mL anhydrous ethanol, and then 0.0636g CsOH-H2O was added to the dissolved; after uniformly dispersed in the mixed solution 10mL of anhydrous acetic acid was added to the above solution, the mixed solution was transferred to a Teflon 100mL autoclave, reacted at 235 ° C 20h, to give a dark blue product was washed with water by centrifugation, washed with ethanol several times, the resulting the product was vacuum dried at 60 ° C for 12h.

 

Cs_xWO₃ nanomaterials capable of catalyzing the oxidation of H₂O₂ substrate to produce color reaction: The Cs_xWO₃ nanomaterials, TMB and H₂O₂ was added to 25 mM, pH = PBS 4.0 buffer solution, the shake, the reaction 30 min, followed by UV spectrophotometry absorbance was measured by 652nm wavelength. Cs_xWO₃  + H₂O₂ + TMB experimental group to other group has a higher absorption values compared to explain Cs_xWO₃ nanomaterials indeed capable of catalyzing the oxidation of H₂O₂ to TMB oxTMB.

 

Aqueous solution of glucose and oxygen occurs in the catalytic reaction of the glucose oxidase produces H₂O₂ can be quantified, and Cs_xWO₃ nanomaterials can catalyze H₂O₂ to produce strong oxidizing hydroxyl radical, then oxidized TMB oxTMB, measured by UV absorbance oxTMB, indirect detection of glucose content in the solution.

 

The present invention first found Cs_xWO₃ nanomaterials catalytically active class peroxidase, and measuring the glucose content of the solution applied. Cs_xWO₃ nanomaterials as a category for the determination of glucose peroxidase, short reaction time, color-fast, etc., the catalytic effect and applicability are higher than the natural peroxidase. And compared to horseradish peroxidase (HRP), it has a simple preparation process, low cost, stable chemical activity, etc., can be used as a substitute for natural peroxidase. And glucose oxidation enzyme-linked with, you can build a simple, fast, low-cost assay for the detection of glucose concentration, the detection limit is 0.1 μ Mo.

 

Compared with horse radish peroxidase (HRP), the Cs_xWO₃ nano material has the advantages of simple preparation process, low cost, stable chemical activity and the like; the catalytic effect and the applicability of the Cs_xWO₃ nano material are higher than those of natural peroxidase, and thus the Cs_xWO₃ nano material can be used as a substitute of natural peroxidase.