Sodium Tungstate Applied in Efficient Production of Phycocyanin

Phycocyanin is a fat-soluble natural carotenoid. Modern science finds that phycocyanin contains a special structure of propadiene and alkylene oxide. This special structure leads to many important physiological activities, such as anti-tumor. Activity, anti-obesity activity, antioxidant activity, regulation of blood sugar, scavenging free radicals, etc., have broad prospects for development in the fields of food, medicine, health care products, and beauty products.

The annual production of Phycocyanin in seaweed can reach 6.8×106 tons. Due to the fact that only in phycocyanin, algae and diatoms, the phycocyaninwas found. The products of phycocyanin are rare in the market due to lack of resources and immature production technology. Therefore, optimizing the purification process of brown algae phycocyanin is conducive to creating greater economic and social significance.

From previous studies on the purification of phycoxanthin from humans, it has been shown that in order to concentrate and more efficiently purify phycocyanin, it is necessary to achieve pre-enrichment of algae in its production process. The key is to control the composition of fatty acids. In the growth environment of algae, nitrogen is an element required for the growth of microalgae. Excessive absorption is likely to cause excessive growth, resulting in consumption or dispersion of phycocyanin, thereby increasing the purification cost.

Sodium tungstate is an inhibitor of nitrate reductase, which is an important regulatory enzyme and rate-limiting enzyme in the process of nitrogen metabolism. It can directly regulate nitrate reduction to regulate nitrogen metabolism, and can inhibit the activity of nitrate reductase, namely tungsten. Sodium can limit the absorption of nitrogen by plants, while nitrogen limitation has an important regulatory effect on total lipids. Therefore, the total lipid content can be adjusted by sodium tungstate.

The researchers used sodium tungstate for the following experiments: 210 ml of sterilized natural seawater were added to each group, 210 μL of MVA mother liquor was added to obtain a nutrient solution, and a nutrient solution volume of one-third of the logarithmic phase was added to the nutrient solution. The algal mother liquor was cultured in a light incubator at 62.5 μmol photons/( m2•s) at 15 °C. Shake the algae evenly in three time periods (8: 00, 12:00, 20:00). After 7 days of culture, the algal cells were collected to determine the fphycocyanin content, and the phycocyanin content was determined to be 10.02 mg/g.

Studies have shown that using sodium tungstate as a regulator, sodium tungstate replaces the molybdenum reductase coenzyme (the active factor that promotes absorption) in the metabolism process, thereby controlling or reducing the activity of nitrate reductase, ultimately inhibiting plant pairs. Absorption of nitrate nitrogen. Through the induction treatment of Phaeodactylum tricornutum, the content of phycocyanin increased significantly, which greatly reduced the cost of phycocyanin production.

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